real-time pcr array (rat inflammatory cytokines receptors rt2 profiler-superarray) Search Results


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DU145 cells were transduced with either Ad-G-AT2R-EGFP (AT2R) or Ad-CMV-EGFP (EGFP) for 2 d at 200 ifu/cell. Other groups of cells were mock transduced. A . Up and down-regulated <t>cytokines</t> were screened by Real-time <t>PCR</t> Array in DU145 cells. Real-time RT-PCR analysis of either IL8 ( B ), IL6 ( C ), BMP6 ( D ), BMP7 ( E ), BMP1 ( F ), BMP4 ( G ), BMP8B ( H ), TGFB1 ( J ), TGFB2 ( J ) and TGFB3( J ). All data were normalized against levels of GAPDH mRNA expression within the same sample. Columns, mean from three separate experiments; bars, SE. *, P < 0.05.
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DU145 cells were transduced with either Ad-G-AT2R-EGFP (AT2R) or Ad-CMV-EGFP (EGFP) for 2 d at 200 ifu/cell. Other groups of cells were mock transduced. A . Up and down-regulated <t>cytokines</t> were screened by Real-time <t>PCR</t> Array in DU145 cells. Real-time RT-PCR analysis of either IL8 ( B ), IL6 ( C ), BMP6 ( D ), BMP7 ( E ), BMP1 ( F ), BMP4 ( G ), BMP8B ( H ), TGFB1 ( J ), TGFB2 ( J ) and TGFB3( J ). All data were normalized against levels of GAPDH mRNA expression within the same sample. Columns, mean from three separate experiments; bars, SE. *, P < 0.05.
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DU145 cells were transduced with either Ad-G-AT2R-EGFP (AT2R) or Ad-CMV-EGFP (EGFP) for 2 d at 200 ifu/cell. Other groups of cells were mock transduced. A . Up and down-regulated <t>cytokines</t> were screened by Real-time <t>PCR</t> Array in DU145 cells. Real-time RT-PCR analysis of either IL8 ( B ), IL6 ( C ), BMP6 ( D ), BMP7 ( E ), BMP1 ( F ), BMP4 ( G ), BMP8B ( H ), TGFB1 ( J ), TGFB2 ( J ) and TGFB3( J ). All data were normalized against levels of GAPDH mRNA expression within the same sample. Columns, mean from three separate experiments; bars, SE. *, P < 0.05.
Rt2 Profiler Pcr Array, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DU145 cells were transduced with either Ad-G-AT2R-EGFP (AT2R) or Ad-CMV-EGFP (EGFP) for 2 d at 200 ifu/cell. Other groups of cells were mock transduced. A . Up and down-regulated <t>cytokines</t> were screened by Real-time <t>PCR</t> Array in DU145 cells. Real-time RT-PCR analysis of either IL8 ( B ), IL6 ( C ), BMP6 ( D ), BMP7 ( E ), BMP1 ( F ), BMP4 ( G ), BMP8B ( H ), TGFB1 ( J ), TGFB2 ( J ) and TGFB3( J ). All data were normalized against levels of GAPDH mRNA expression within the same sample. Columns, mean from three separate experiments; bars, SE. *, P < 0.05.
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DU145 cells were transduced with either Ad-G-AT2R-EGFP (AT2R) or Ad-CMV-EGFP (EGFP) for 2 d at 200 ifu/cell. Other groups of cells were mock transduced. A . Up and down-regulated <t>cytokines</t> were screened by Real-time <t>PCR</t> Array in DU145 cells. Real-time RT-PCR analysis of either IL8 ( B ), IL6 ( C ), BMP6 ( D ), BMP7 ( E ), BMP1 ( F ), BMP4 ( G ), BMP8B ( H ), TGFB1 ( J ), TGFB2 ( J ) and TGFB3( J ). All data were normalized against levels of GAPDH mRNA expression within the same sample. Columns, mean from three separate experiments; bars, SE. *, P < 0.05.
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DU145 cells were transduced with either Ad-G-AT2R-EGFP (AT2R) or Ad-CMV-EGFP (EGFP) for 2 d at 200 ifu/cell. Other groups of cells were mock transduced. A . Up and down-regulated <t>cytokines</t> were screened by Real-time <t>PCR</t> Array in DU145 cells. Real-time RT-PCR analysis of either IL8 ( B ), IL6 ( C ), BMP6 ( D ), BMP7 ( E ), BMP1 ( F ), BMP4 ( G ), BMP8B ( H ), TGFB1 ( J ), TGFB2 ( J ) and TGFB3( J ). All data were normalized against levels of GAPDH mRNA expression within the same sample. Columns, mean from three separate experiments; bars, SE. *, P < 0.05.
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DU145 cells were transduced with either Ad-G-AT2R-EGFP (AT2R) or Ad-CMV-EGFP (EGFP) for 2 d at 200 ifu/cell. Other groups of cells were mock transduced. A . Up and down-regulated <t>cytokines</t> were screened by Real-time <t>PCR</t> Array in DU145 cells. Real-time RT-PCR analysis of either IL8 ( B ), IL6 ( C ), BMP6 ( D ), BMP7 ( E ), BMP1 ( F ), BMP4 ( G ), BMP8B ( H ), TGFB1 ( J ), TGFB2 ( J ) and TGFB3( J ). All data were normalized against levels of GAPDH mRNA expression within the same sample. Columns, mean from three separate experiments; bars, SE. *, P < 0.05.
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DU145 cells were transduced with either Ad-G-AT2R-EGFP (AT2R) or Ad-CMV-EGFP (EGFP) for 2 d at 200 ifu/cell. Other groups of cells were mock transduced. A . Up and down-regulated cytokines were screened by Real-time PCR Array in DU145 cells. Real-time RT-PCR analysis of either IL8 ( B ), IL6 ( C ), BMP6 ( D ), BMP7 ( E ), BMP1 ( F ), BMP4 ( G ), BMP8B ( H ), TGFB1 ( J ), TGFB2 ( J ) and TGFB3( J ). All data were normalized against levels of GAPDH mRNA expression within the same sample. Columns, mean from three separate experiments; bars, SE. *, P < 0.05.

Journal: PLoS ONE

Article Title: Gene Expression Profiling Associated with Angiotensin II Type 2 Receptor-Induced Apoptosis in Human Prostate Cancer Cells

doi: 10.1371/journal.pone.0092253

Figure Lengend Snippet: DU145 cells were transduced with either Ad-G-AT2R-EGFP (AT2R) or Ad-CMV-EGFP (EGFP) for 2 d at 200 ifu/cell. Other groups of cells were mock transduced. A . Up and down-regulated cytokines were screened by Real-time PCR Array in DU145 cells. Real-time RT-PCR analysis of either IL8 ( B ), IL6 ( C ), BMP6 ( D ), BMP7 ( E ), BMP1 ( F ), BMP4 ( G ), BMP8B ( H ), TGFB1 ( J ), TGFB2 ( J ) and TGFB3( J ). All data were normalized against levels of GAPDH mRNA expression within the same sample. Columns, mean from three separate experiments; bars, SE. *, P < 0.05.

Article Snippet: The cDNA samples were also screened for the expression of 84 pathway-focused cytokines and by means of RT 2 Profiler PCR Array Human Common Cytokines (PAHS-021A; Superarray, Frederick, MD, USA) on an ABI 7500 real-time PCR system (Applied Biosystems, Carlsbad, CA, USA), according to the manufacturer’s protocols.

Techniques: Transduction, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing