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Image Search Results
Journal: PLoS ONE
Article Title: Gene Expression Profiling Associated with Angiotensin II Type 2 Receptor-Induced Apoptosis in Human Prostate Cancer Cells
doi: 10.1371/journal.pone.0092253
Figure Lengend Snippet: DU145 cells were transduced with either Ad-G-AT2R-EGFP (AT2R) or Ad-CMV-EGFP (EGFP) for 2 d at 200 ifu/cell. Other groups of cells were mock transduced. A . Up and down-regulated cytokines were screened by Real-time PCR Array in DU145 cells. Real-time RT-PCR analysis of either IL8 ( B ), IL6 ( C ), BMP6 ( D ), BMP7 ( E ), BMP1 ( F ), BMP4 ( G ), BMP8B ( H ), TGFB1 ( J ), TGFB2 ( J ) and TGFB3( J ). All data were normalized against levels of GAPDH mRNA expression within the same sample. Columns, mean from three separate experiments; bars, SE. *, P < 0.05.
Article Snippet: The cDNA samples were also screened for the expression of 84 pathway-focused cytokines and by means of
Techniques: Transduction, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing